This protocol has been developed to help to overcome functional B2 deficiency in
conditions such as Chronic Fatigue Syndrome and Autism Spectrum Disorder, where
it is commonly seen that the functional B2 deficiency is reducing the
effectiveness of vitamin B12 supplementation. The protocol could equally well be
used in Paradoxical B12 deficiency, as well as Diabetes, Obesity, Dementia, CVD,
cachexia (muscle wasting) hypertension, or other conditions associated with either functional B2
deficiency or Paradoxical B12 deficiency (Paradoxical).
The use of the protocol is a preparatory step before injection or transdermal
application of vitamin B12.The protocol is the property of B12
Oils Pty Ltd.
It has been known for over 40 years, that active vitamin B2 (as FMN and FAD) are
essential to maintain the activity of vitamin B12, and very commonly it has been
found that functional vitamin B12 deficiency is caused by lack of
functional vitamin B2 (as FMN and FAD), which has been caused by a lack of
vitamin B2 per se, and a possible lack of Iodine, Selenium and/or Molybdenum,
all of which are required for the activation of vitamin B2. Riboflavin (vitamin
B2) is inactive unless it is first activated within the cell. This activation
requires three specific steps, which require Iodine, Selenium and Molybdenum. The protocol revolves around a very gradual introduction of Iodine, Selenium and
Molybdenum. Introduction is slow as in extreme deficiency simultaneous administration of all three
can have strong side effects. Supplementation with vitamin B2 alone will not
work, unless there is sufficient Iodine, Selenium and Molybdenum.
The protocol assumes that you are low on all of Iodine, Selenium and Molybdenum,
which can be assessed by analysis of HMTA and/or thyroid markers. If you are
already sufficient in Iodine, or Selenium or Molybdenum, as judged by these
markers, then you do not need to necessarily supplement the mineral you are sufficient in.
Everyone, regardless, does require at least the Recommended Daily Allowance
(RDA) or Recommended Daily Intake (RDI) of Iodine, Selenium and Molybdenum. You
need this each and every day of your life or you become deficient. This
means everyday, for ever more, not just at the time of the protocol, but
on-going, basically until you stop breathing. If
you do not get the RDA for Iodine, Selenium and Molybdenum then the activation of vitamin B2 will be compromised. The
ranges listed represent minimum and maximum ranges of daily intake for a person
who is replete in Iodine, Selenium and Molybdenum. The protocol assumes that you
remain on the same brand of supplement, and that you do not change mid-way, if
you do, you may have to start titrating up again. If you go up a dose and you
get a strong reaction, you should go back to the dose that you tolerated and
make the incremental increase smaller.
Iodine is at the top of the vitamin B2 activation cascade, as pictured below.
(Iodine - I, Selenium - Se, Molybdenum - Mo)
Start with 50 ug/day for one week and increase to 100 ug/day, week 2. Week 3 and
ongoing 150 ug/day. The iodine should be as either the Sodium or Potassium
Iodide salt. Lugol's Iodine does not work in this protocol. A suitable
topical product is available at
https://b12oils.com/order.htm
Natural sources of Iodine are: Seafood, Dairy, Eggs, Grains, and fortified
products such as Iodized bread and Iodized salt. You
should avoid goitrogens, such as soy, Cassava, Broccoli, Cauliflower,
Cruciferous veggies, and Kale.
Symptoms of over-supplementing with Iodine are generally flu-like symptoms,
drowsiness, brain fog following Iodine supplementation, and a drop in TSH below
0.5. RDA for Iodine is 150-300 ug/day (each and every day). Care
must be taken not to over-supplement with Iodine, as this can lead to autoimmune
thyroiditis (AIT), in which thyroid autoantibodies are elevated.
Over-consumption of high iodine containing seaweed has lead to AIT, as has
consumption of Iodine-containing multivitamins which have contained much higher
levels of Iodide than stated on the label. Our
data suggests that a suitable target for Iodine can be assessed by monitoring
TSH, where a target of between 0.5 and 1.5 should be aimed for. This is in
agreement with suggestions made by Kelly (2018) and Cohen and co-workers (2018).
The RDA for Selenium is 55-200 ug/day, and the aim is to achieve this. In
children under 10 aim for 55ug, for adults 200 ug.
After 2 weeks of Iodide supplementation (week 3), continue with the Iodide and
add 25 ug/day Selenium and slowly build to 200 ug/day. The selenium should be as
the Selenite, or Selenate salt, but should not be Selenomethionine or Selenium
chelates, or Selenium amino acids complex, each of which has been shown not to
work. Start with 25 ug/day for one week and increase to 50 ug/day, week 2. Week
3, 100 ug/day, with week 4 and ongoing 200 ug/day. Adjust doses for children.
Some persons may find some of the increases too much, in which case you would
need to reduce the size of the increase. If you switch from Selenomethionine to
Selenite, you would have to start at the lowest dose, as the efficacy of Selenomethionine is minimal.
A suitable topical product is available at
https://b12oils.com/order.htm
Natural sources of Selenium are: Eggs, Tuna, Shrimp, Beef, Turkey, and wheat
from Selenium sufficient soils. Organic foods or gluten-free foods are often low
in Selenium as too wheat from selenium depleted soils such as the UK, much of
Europe, New Zealand, much of Australia and many States in the USA.
Avoid administering any product with diatomaceous earth, fulvic acid, large dose
vitamin C, as they can reduce absorption of Selenite (Robinson etal, 1985; Ip,
1986). Further do not administer any product together with calcium or magnesium
as they will bind to the Selenite and make it non-bioavailable. The
RDA for Molybdenum is 100-300 ug/day. This is addition to Iodine, and Selenium. At
week 4, continue with the Iodine and Selenium. Start with 50 ug/day Molybdenum
(as the Molybdate salt) and gradually increase to at least 200 ug/day. Some
adults have been found to need as much as 300 ug/day. The
Molybdenum should NOT be a Molybdenum chelate or Molybdenum or Molybdate chelate
or Molybdenum amino acids complex. Half the doses for children under 10. A
suitable topical product is available at
https://b12oils.com/order.htm Natural sources of Molybdenum are lentils, peas, beans, oats, barley, bread,
pasta, but only if they are grown in Molybdenum sufficient soils that are basic.
Avoid administering any product with diatomaceous earth, fulvic acid, large dose
vitamin C, as they can reduce absorption of Molybdate (Robinson etal, 1985; Ip,
1986). Further do not administer any product together with calcium or magnesium
as they will bind to the Molybdate and make it non-bioavailable. >At
week 4 you can slowly start to introduce vitamin B2. Start with 2 mg/day for
week 4, 5 mg/day for week 5 and 10 mg/day ongoing (after week 6). You must keep
going with the Iodine, Selenium and Molybdenum. Once
the Iodine/Selenium and Molybdenum and B2 have been introduced (at around week
6), then you can start on the mixed Adenosyl/Methyl B12 oil mixture (see
https://b12oils.com/order.htm ).
Start with just a drop of oil from a dropper. Apply the oil into an area of
clean dry skin and rub it in like a massage oil. If the child/adult can tolerate
the drop, then on the following day increase to 2 drops and gradually increase
to a whole squirt/dose. NB you cannot get enough vitamin B12 to restock the
brain of a child or an adult from any oral supplement, whether it be lozenge,
high dose oral or even nasal delivery. Further, the effect of doing so will take
time to become apparent. Myelination is a slow process. As
the protocol starts to work, levels of serotonin and dopamine that have been
over-produced due to the vitamin B12 deficiency may start to drop down to what
should eventually be normal levels. For example a twenty-fold increase in
serotonin is not uncommon in B12 deficiency, and levels at much as 200 times
normal have also been noted. As the body starts to fix B12 deficiency, these
levels can drop precipitously. The body can take a long time to adapt to these
new "normal" levels and in some that can lead to feelings of depression and
anxiety. This is indicative that you are starting to fix functional B2 and then
B12 deficiency, and that your body is returning to normal levels. If at this
time you feel you need help, you should make sure you have good emotional
support. A brief description of what is going on is at
https://b12oils.com/receptors.htm
Most
children or adults on GFCF diets are vitamin B1 deficient (as too B2/I/Se/Mo and
Calcium). Further B2 deficiency begats B1 deficiency. Vitamin B1 should be
introduced at around week 6.
Early on in the protocol, avoid folate until the B2 and B12 have been optimized.
You can introduce folate (either as folic acid, folinic acid or 5MTHF - 400
ug/day). You can and should try to get folate naturally in the diet by eating
leafy green veggies. This will also provide the essential mineral magnesium. Many
people with ASD or CFS believe that the condition is due to an allergy to eggs,
and so avoid them. In this case they will more than likely be biotin deficient.
Supplement with 150 ug/day - or introduce eggs once the protocol has been
established. Iron
deficiency is extremely common in ASD. For neurotypically normal development
large quantities of iron are required for the process of myelination of the
brain and peripheral nervous system. Estimates are 7 mg/day for children 1 to 8
years old. This estimate is of bioavailable iron, NOT of iron in the supplement. It is best to introduce iron containing
meats, such as beef or chicken liver, clams, mollusks or mussels or oysters >>
beef, lamb goat, deer, bison, sardines turkey, all of which have much higher
iron contents than chicken or pork. Non-meat sources of iron are extremely
poorly absorbed. Buy
a multivitamin that has all of Iodine (as Iodide 150 ug), Selenium (as Selenite
55-200 ug) and Molybdenum (as Molybdate 100-300 ug). Start with a low dose
(around 1/8th of the above) and slowly increase to a full dose over a period of
about 4 weeks. It should be noted that you will also be slowly increasing the
dose of vitamin B2, along with the I/Se/Mo. A mixed product is available at
https://b12oils.com/order.htm It
is quite hard to find a suitable multivitamin that has all of Iodine (as Iodide
150 ug), Selenium (as Selenite 55-200 ug) and Molybdenum (as Molybdate 100-300
ug) in the right forms, and many will require additional supplementation with
Iodide, Selenite, or Molybdate.
Iodine should be as the sodium or potassium Iodide salts.
https://shop.bodybio.com/collections/liquid-mineral/products/iodine-9-liquid-mineral-2oz
https://au.iherb.com/pr/Nature-s-Plus-Potassium-Iodide-150-mcg-100-Tablets/42445
https://au.iherb.com/pr/World-Organic-Liquid-Potassium-Iodide-2-fl-oz-59-ml/7775
Benevolent Iodine -
potassium Iodide 100 ug/drop
https://www.pureformulas.com/selenium-solution-8-fl-oz-236-ml-liquid-by-allergy-research-group.html
https://au.iherb.com/pr/nutricology-selenium-solution-8-fl-oz-236-ml/17257
https://www.chempro.com.au/NUTRITION-CARE-SODIUM-SELENITE-DROPS-50ML
http://www.iherb.com/Eidon-Mineral-Supplements-Selenium-Liquid-Concentrate-2-oz-60-ml/26013
https://www.amazon.com/dp/B0002UIRBM/ref=psdc_3774471_t1_B000IRJOZ2
https://www.amazon.com/gp/product/B00024D52Y/ref=oh_aui_search_detailpage?ie=UTF8&psc=1
https://shop.bodybio.com/collections/liquid-minerals/products/selenium-8-liquid-mineral-2oz
https://www.fair-pure.com/en/selen-50g-from-sodium-selenite-vegan
NB only sodium and ammonium molybdate supplements work, as
Molybdenum is internalized via a molybdate transporter, and so other forms of
Molybdenum do not work (Mendel and Bittner, 2006)
https://www.metabolics.com/sodium-molybdate-pot-of-90-capsules.html
https://www.fair-pure.com/en/molybdenum-150g-120-tablets-molybdate-vegan
https://www.biocare.co.uk/nutrisorbr-liquid-molybdenum-15ml
https://www.epigenetics-international.com/product/molybdenum-100ml
https://www.b12oils.com/order.htm
Molybdenum - NaMolybdate 300 ug Molybdate / 8 drops. Transdermal.
Combined Iodide/Selenite/Molybdate
https://www.b12oils.com/order.htm
ISeMo Iodine - NaIodide (150 ug), Selenium - NaSelenite (55 ug), Molybdenum -
NaMolybdate (100 ug)
https://www.naturesway.com/Product-Catalog/Alive-Once-Daily-Women-s-50-Ultra-60-Tabs
KI(150 ug), NaSelenate (250 ug) NaMolybdate (75 ug).
https://www.biocare.co.uk/methyl-multinutrient KI (200ug), NaSelenite (200
ug), NaMolybdate (200 ug) MeCbl (400 ug) The start of methylation can sometimes induce a higher than
normal production of Adenalin, which can cause some odd side-reactions, such as Increased fatigue, joint or muscle
pain, skin rashes, photosensitivity, irritability, paresthesia, dizziness, sleep
disturbances, asthenia, muscle cramps, night sweats, hypertension, hypotension,
headaches (especially migraines) and swollen glands. Also reported are heavy
perspiration, metallic taste in mouth, chills, nausea, bloating, constipation or diarrhea, low grade fever, heart palpitations, tachycardia, facial palsy,
tinnitus, mental confusion, uncoordinated movement, pruritus, bone pain,
flu-like syndrome, conjunctivitis and throat swelling" If the person attempting the protocol has these symptoms during the dose
escalation stage of the protocol, go back to the previous dose for a few days
before increasing the dose once more. Be aware that children with autism cannot
tell you what these symptoms. Further they have no idea of what is causing the
symptoms or how to tell you. The most likely symptom is a rise in blood
pressure, which may cause them to experience head-aches. Once the person or child has been on the vitamin B2/I/Se/Mo
supplementation combined with the mixed Adenosyl/Methyl B12 oils for two months,
the urinary Organic Acids test should be repeated in order to check that the
regime is working. At this time Contact us and we can
help determine if the protocol has been effective. Tolerable Upper limit Iodine Iodine deficiency >50% of population,
150-300 ug/day more likely if no dairy. Hypothyroidism with <20 ug/day Seafood, Dairy, eggs, grains Goitrogens: Soy, cassava, cabbage, Chard, broccoli, cauliflower, cruciferous veggies.
` Deficiency of iron or vitamin A Selenium Tolerable Upper limit - 200 ug 55 -200 ug/day Tuna, Ham, Shrimp, Beef, Turkey, Chicken, eggs. Grains, depending upon where they come
from. Brazil Nuts have selenomethionine, not selenocysteine. Molybdenum Tolerable Upper limit - 2 mg 100 -300 ug/day Lentils, peas, beans, oats, Barley, Bread, Pasta, Rice NB. Most
gluten-free products are deficient in Iodine, Selenium and Molybdenum. Further
they are not fortified with either folate or vitamin B1. Additionally they often
contain high levels of arsenic, lead, and mercury, which is readily seen by
increases in values in Hair Metals Test Analysis
More about vitamin B2 and the effect of deficiency can be found at
https://lpi.oregonstate.edu/mic/vitamins/riboflavin#deficiency-risk-factors
The most readily absorbed form of iron is heme iron, from sources such beef,
goat, lamb, squid, octopus, mussels, oysters and clams etc. Non-heme iron, such
as the iron in quoted vegetables such as soy, lectins, spinach, etc are very
poorly absorbed and have not been found to significantly raise serum ferritin
levels. Potential
supplements Ortho Iron
(Iron-ferric pyrophasphate/lactoferrin (30 mg) lactoferrin (100 mg), Copper (0.9
mg)
https://au.iherb.com/pr/advanced-orthomolecular-research-aor-ortho-iron-60-vegetarian-capsules/73593
ProFerrin ES90 10.5 mg Heme iron
https://proferrin.com/international-distribution Plain B group
supplement Klaire.com B comples
plus
https://klaire.com/v803-10-b-complex-plus Supplements
containing Iodide, Selenite, and Molybdate are rather hard to find. Some are
listed below:
https://au.iherb.com/pr/Nature-s-Way-Alive-Once-Daily-Women-s-Ultra-Potency-Multi-Vitamin-60-Tablets/39614
KI 150 ug, NaSelenate 250 ug, NaMolybdate 75 ug.
https://www.seroyal.com/trace-mineral-comple-cws.html
KI 50 ug, NaSelenate 50 ug, NaMolybdate 50 ug.
https://www.biocare.co.uk/methyl-multinutrient KI (200ug), NaSelenite (200
ug), NaMolybdate (200 ug) MeCbl (400 ug)
https://www.awakennutrition.com/supplement-facts/ KI(100 ug) NaSelenite (50
ug), NaMolybdate (100 ug) MeCbl (1000 ug).
NB, Oral MeCbl in high doses is inactivated in the stomach, so although it may
be present in a supplement, its utility in any oral supplement is questionable.
https://au.iherb.com/pr/Nature-s-Way-Alive-Once-Daily-Women-s-Ultra-Potency-Multi-Vitamin-60-Tablets/39614
KI 150 ug, NaSelenate 250 ug, NaMolybdate 75 ug.
https://www.biocare.co.uk/methyl-multinutrient KI (200ug), NaSelenite (200
ug), NaMolybdate (200 ug) MeCbl (400 ug)
https://www.naturesway.com/Product-Catalog/Alive-Once-Daily-Men-s-Ultra-60-Tabs
KI 150 ug, NaSelenate 250 ug, NaMolybdate 75 ug. CN Cbl 100ug
Calcium 130 mg
https://www.awakennutrition.com/supplement-facts/ KI(100 ug) NaSelenite (50
ug), NaMolybdate (100 ug) MeCbl (1000 ug).
https://www.bioticsresearch.com/node/1526
https://www.seekinghealth.com/products/molybdenum-90-capsules
https://www.allergyresearchgroup.com/liquid-molybdenum-1-fl.-oz.-30-ml
https://shop.bodybio.com/collections/liquid-minerals/products/molybdenum-7-liquid-mineral-2oz
https://www.biocare.co.uk/selenium
- It is Selenomethionine, not Selenite
https://www.nutricology.com
Nutricology Liquid Molybdenum - has sodium molybdate, BUT, also has citric acid
in it, which reduces Molybdate.
Genestra Trace Minerals complex - contains Chromium, which has been shown to
cause flushing reactions on the skin in many individuals.
Mendel and Bittner,
2005 Kelly, TM, 2018 The
Art and Science of Thyroid Supplementation for The Treatment of Bipolar
Depression (Book) Robinson etal 1985
Effect of megadose of ascorbic acid, a meal and orange juice on the absorption
of selenium as sodium selenite. NZ Med J. 98: 627-9
Cohen et al.Antidepressant-Resistant Depression in
Patients With Comorbid Subclinical Hypothyroidism or High-Normal TSH Levels.Am J
Psychiatry. 2018 Jul 1;175(7):598-604. doi: 10.1176/appi.ajp.2017.17080949.
Copyright © 2018 B12 Oils. All Rights Reserved.
RnB Treatment Protocol
Riboflavin
and B12 treatment
Caution
First start
by introducing Iodine
Iodine
build
Selenium
build
Molybdenum
build
Vitamin B2
(riboflavin) build
Vitamin B12
(Adenosyl/Methyl B12) build
Depression and Anxiety
Vitamin B1
Deficiency
Folate
Deficiency
Biotin
Deficiency
Iron
Deficiency
Alternative
Protocol
Potentially
suitable supplements
Iodine Supplements
Selenium Supplements
Molybdenum Supplements
Multivitamin Supplements
Multivitamin Supplements
Side Reactions
Follow Up
Natural
food sources of Iodine, Selenium and Molybdenum
AVOID
Role of Vitamin B2
Iron Supplements
General Supplements
Supplements that may not work due to Calcium and Magnesium
content
Supplements that have been shown NOT to work
Supplements with undesirable side-effects
References
Ip C. Interaction of vitamin C and selenium supplementation in the modification
of mammary carcinogensis in rats. J. Natl. Cancer Intst 1986 77:299-303
PMID 3088312
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