Research Findings - USA

We have been retrospectively examining data obtained from parents of children who have autism/developmental delay, to see if there is any basic differences in the Biochemistry between countries. We have separated out the Hair Metals Test Analysis (HMTA) data and Organic Acids Test (OAT) data, and present the findings in graphical form below.

HMTA

Previous data has shown that in Modern Day Autism, the major precipitating factor is functional B2 deficiency due to deficiencies in Iodine, Selenium and/or Molybdenum, all of which are required to activate vitamin B12. We present this data below. As can be seen all of the children were deficient in one or more of Iodine (<1.0 ppm : 15/18), Selenium (<1.0 ppm 14/19), or Molybdenum (<0.08 ppm: 13/19), with dual deficiencies being common. Hence, the data would suggest that these children would have functional vitamin B2 deficiency. The observation of Iodine deficiency in the children is consistent with data on the known Iodine deficiency in USA. It is becoming increasingly obvious that deficiency of Selenium and/or Molybdenum is now more common, with resultant lack of activation of vitamin B2. Hence deficiency of any of I/Se/Mo in the soil or diet is causing functional B2 in mothers or children resulting in functional B12 deficiency with developmental delay being the consequence.

OAT

We present this data below.

All children had functional deficiency in Adenosyl vitamin B12, with elevated MMA being common and elevated methylsuccinate universal.

Functional vitamin B2 deficiency was seen in all the children, with various levels of elevated oxalate, lactate, and succinate.

Consistent with data from many other countries, children in USA, generally were iron deficient, suggesting that the observed functional B12 deficiencies seen were a combination of low dietary intake of iron by the mothers as well as functional B2 deficiency. Elevated citrate occurs due to uncoupling of aconitase when ferritin is below 60 ug/L.

Vitamin B1 deficiency was common (9 of 10), as judged by elevated AKG, and elevated pyruvic acid. This is commensurate with a gluten-free diet, which was common in the children.

All children were functionally deficient in Methyl B12 as judged by VMA, HVA, 5HIAA, QA, and KA, with elevated pyroglutamic acid being common.

Data suggests that in deficiency of Iodine and/or Molybdenum QA:KA ratio increases, succinate increases, as too pyroglutamate, due to lack of formation of FMN from riboflavin.

All children showed some degree of vitamin D deficiency as judged by elevated phosphoric acid.

Publications

Stoeva I, Peneva L, Grigorova R, Vassileva B, Brumm H, Grüters A. Neonatal TSH screening--an instrument of iodine supplementation monitoring in Bulgaria in comparison to Berlin--a preliminary report. Exp Clin Endocrinol Diabetes. 1997;105 Suppl 4:51-4. doi: 10.1055/s-0029-1211933. PMID: 9439916.

Delange F. Iodine deficiency in Europe and its consequences: an update. Eur J Nucl Med Mol Imaging. 2002 Aug;29 Suppl 2:S404-16. doi: 10.1007/s00259-002-0812-7. Epub 2002 Jun 1. PMID: 12192540.

Møllehave LT, Eliasen MH, Strēle I, Linneberg A, Moreno-Reyes R, Ivanova LB, Kusić Z, Erlund I, Ittermann T, Nagy EV, Gunnarsdottir I, Arbelle JE, Troen AM, Pīrāgs V, Dahl L, Hubalewska-Dydejczyk A, Trofimiuk-Müldner M, de Castro JJ, Marcelino M, Gaberšček S, Zaltel K, Puig-Domingo M, Vila L, Manousou S, Nyström HF, Zimmermann MB, Mullan KR, Woodside JV, Völzke H, Thuesen BH. Register-based information on thyroid diseases in Europe: lessons and results from the EUthyroid collaboration. Endocr Connect. 2022 Mar 10;11(3):e210525. doi: 10.1530/EC-21-0525. PMID: 35044931; PMCID: PMC8942317.

Alehagen U, Aaseth J. Selenium and coenzyme Q10 interrelationship in cardiovascular diseases--A clinician's point of view. J Trace Elem Med Biol. 2015;31:157-62. doi: 10.1016/j.jtemb.2014.11.006. Epub 2014 Nov 27. PMID: 25511910.